2nd International Probiotics, Nutrition & Microbiome Conference
October 10-11, 2018 Amsterdam, Netherlands
Bacteriocinogenic Lactobacillus plantarum Inhibit Adhesion of Gastrointestinal Pathogens onto Extracellular Matrix and Intestinal Cells
Indian Institute of Technology Guwahati, India
Probiotic lactic acid bacteria (LAB) can be explored to develop a safe and niche-specific therapeutic regimen against bacterial infections based on their ability to thwart adhesion of pathogens onto intestinal cells. The present investigation reports the potential of native Lactobacillus plantarum strains to inhibit adhesion of model gastrointestinal pathogens, Enterococcus faecalis and Staphylococcus aureus on extracellular matrix (ECM) and cultured intestinal cells (HT-29 cells). A native L. plantarum CRA21 strain rendered significant inhibition of S. aureus MTCC 96 and E. faecalis MTCC 439 adhesion onto ECM molecules, collagen and mucin, with the highest inhibition of S. aureus MTCC 96 adhered on collagen observed in the exclusion mode (83.61%) as compared to competition (62.4%) and displacement (38.58%) mode of adhesion assay. A dual-color flow cytometry (FCM) based adhesion assay indicated that in the exclusion mode, L. plantarum DF9 rendered notable inhibition of pathogen adhesion onto HT-29 cells, with the relative adhered population of E. faecalis MTCC 439 being 3.94% and that of L. plantarum DF9 being 77.56%. FCM along with principal component analysis (PCA) revealed that the native strains of L. plantarum DF9 and L. plantarum CRA38 could significantly affect both the adhesion process parameters kd and em of pathogen, similar to the standard probiotic L. rhamnosus GG. Interestingly, addition of the bacteriocin plantaricin A obtained from L. plantarum could reduce the viability of ECM-adhered pathogens. Host cell adhesion assays indicated that addition of plantaricin A on LAB and pathogen adhered onto HT-29 cells led to a prominent reduction in the adhered E. faecalis MTCC 439 cells (17.4%) as compared to L. plantarum DF9 (70.5%), which highlighted the benefit of using LAB bacteriocin for selective eradication of pathogen and minimal collateral damage. It is envisaged that the native probiotic L. plantarum strains can be used in conjunction with LAB bacteriocins as niche-specific anti-adhesion therapeutic agents against gastrointestinal pathogens.
Sandipan Mukherjee is a doctoral student in the Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India. During his doctoral tenure, he has essentially ascertained the probiotic attributes of native lactic acid bacteria (LAB) and its secreted bacteriocin in inhibiting pathogen adhesion onto extracellular matrix and cultured human intestinal cells.