International Probiotics and Antimicrobial Proteins Conference
November 6-8, 2017 | Barcelona, Spain
Encapsulation of Pediococcuspentosaceus OZF, A Promising Probiotic Bacterium
1Ankara University, Department of Biology, Turkey
2Muş Alparslan University, Department of Molecular Biology and Genetics, Turkey
In this study, a promising probiotic bacterium Pediococcuspentosaceus OZF, isolated from human breastmilk in our previous studies was encapsulated with calcium alginate (2%) andwhey (5%) proteins, followed by lyophilization. Surface morphologies of the prepared microcapsules were analyzed under scanning electronmicroscope. Alginate capsules containing OZF cells were coated with whey proteins and were found to be stable at 4°C for 6 months without loss of any viability, and encapsulated OZF was found to be viable in simulated gastric and intestinal juice conditions such as pH, bile salts, pepsin and pancreatin. Transit of capsulated and noncapsulated OZF through gastrointestinal system (GIS), their adhesions as well as their effects on faecal microbiota variations werea nalyzed on murine model (Balb/C mice) by microbiological counts, qPCR and DGGE, respectively. OZF strain was detected in feacalsamples of animal followed by 6 hours after feeding, reached the highes tvalue in 10thhours and started to decrease after and lost completely at 24 hr. At 24 hours following the 15thday of feeding, the number of bacteria adhered to the intestine was determined. At the beginning of the trial, 8 log cfu was applied per animal and 2 log was found in the group feed with nonencapsulated OZF, which means there was 6 log decrease in the adhesion of OZF to GIS at 24 hr. Adhesion properties of encapsulated strain was also found to be the same (2.5log, p>0.05). As far as the feacal microbial composition on the general microbiota is concerned in terms of dominant groupssuch as Bifidobacteria, Lactobacillus, E.coli and Bacterioides, no significal diffecences were recorded between control and experimental groups.
Harun Onlu is graduated from the Biology department of Yuzuncu Yıl University and he completed his master degree in the same university. Later, he was started his PhD in Biology Department of Ankara University in 2011 under the supervision of Prof. Dr. OzlemOsmangaoglu. During the years between 2011-2016, he worked as a research assistant in this department. While he was doing his PhD, he went to Belgium as a researcher in the University catholique de Louvain, Institute of life science Pascal Hols group by getting scholarship fromthe Scientific and Technological Research Council of Turkey (TUBİTAK) between the years of 2015-2016, to carry out some experiments of his PhD thesis. His PhD works are still continuing. He is currently research assistant in theMuşAlparslan University, Department of Molecular Biology and Genetics. His working areas are about probiotic bacteria, bacteriocins, molecular genetics, cloning and knock-out of lactic acid bacteria and lactic acid production.