International Conference on Food Science and Bioprocess Technology
November 20-22, 2017 Dubai, UAE
Antioxidant, Antidiarrhoeal & Anticancerous Effect of Nanocoated Food Packages (Glycyrrhiza glabra)
Avinashilingam Institute for Home Science and Higher Education for Women, India
The commonly used polycarbonate bottles contain Bisphenol A (BPA). Exposure to BPA causes cancer and mimics the hormonal system in pregnant women and children. Only recently, the polycarbonate bottles are banned, in India. As an alternate to BPA, Polypropylene (PP) is used in baby bottles, drinking straws and microwavable wares. The major chemical migrants in PP namely, Octa decyl 3(3-5 di-tert-butyl-4-hydroxy phenyl) and propionate/ Iganox 1076 are known to cause cancer of liver, kidney and lungs in experimental animals. Hence the present study was conducted to synthesize a protective coating from four medicinal plants, of which Glycyrrhiza glabra was found to possess the highest antioxidant, antimicrobial and anticancer properties. The particle size, zeta potential and EDAX of nanoparticles from Glycyrrhiza glabrawere 34.7nm, -24.7mV and 2.60 percent of silver respectively. These properties along with the phytochemicals, augmented the chelating and antimicrobial potentialities of the plant extract. Thin layer chromatography of Glycyrrhiza glabra showed the presence of phenolic compounds and antioxidant activity of 520 µg. FTIR results revealed chlorohexidine salts peaks at 1157 cm-1, which indicate breaking up of lipopolysaccharide. Chlorohexidine is a cationic biocide that damages the outer membrane of gram negative bacteria. Secondly the peak 1442.72 cm-1 corresponds toC=O stretch which shows remarkable intensity changes, revealing better antimicrobial potentials of the encapsulated nanoparticle compared to the raw plant extract. The GC/MS identified the following compounds namely tetradecanoic acid, Z, Z 2,5 pentadecadiene-1-ol, cis-9,10 epoxy octadecan-1-ol, 9 octadecenal and hexadecanoic acid.Hence, Glycyrrhiza glabra nanoparticles were coated onto the feeding bottles and the foods stored in them were tested for their shelf-life for three hours. Nano coats of Glycyrrhiza glabra were also tested on pet bottles and ziplock covers and foods tested over 10 days. Milk stored in feeding bottles recorded a maximum microbial inhibition of 84 percentage and diluted lemon juice showed no microbial growth of S.dysenteriae. On 10th day, nanocoated zip lock covers and PET bottles showed a maximum microbial inhibition of 38, 63 and 83 percent and 57, 68 and 88 percentage against E.coli(MTCC 40), S.enterica (MTCC 3219) and S.dysenteriae (PSGIMS & R) respectively. The MTT assay of Glycyrrhiza glabra nanoparticles showed a maximum apoptosis of 50 and 48 percent against Hela and liver carcinoma cell lines respectively. The gel electrophoresis has led to a longer tail DNA damages with the Hela and carcinoma cell lines, compared to the control and marker. Therefore, nanocoats of Glycyrrhiza glabra could be used for effective protection against oxidative damage, diarrhea and cancer.
Dr. M. Sylvia Subapriya is a Professor in Department of Food Science and Nutrition having 24 years of teaching and research experience and is NSS Programme Coordinator, Avinashilingam University, Coimbatore. She has guided about 4 Ph.D. scholars and 60 post graduate students. To her credit, she has completed 3 national projects and Unicef SMART Survey. She has authored chapters in 2 books and published 12 international and 20 national level articles in refereed journals. Has also participated and presented papers in conferences at Kuantan and Kuala Lumpur, Malaysia. She is on the Editorial Board of the International Journal of Sports Sciences and Recreation, Malaysia Member in various committees and boards of national standards like Nutrition Society of India, Home Science Association of India, Indian Science Congress and Indian Dietetic Association.