Madridge Journal of Food Technology

ISSN: 2577-4182

International Conference on Food Science and Bioprocess Technology

November 20-22, 2017, Dubai, UAE
Scientific Session Abstracts
DOI: 10.18689/2577-4182.a1.010

Ambient Mass Spectrometry for Food Science and Industry

Konstantin Chingin1 and Huanwen Chen2

1East China University of Technology, China
2Jilin University, China

Requiring little or no sample pretreatment, ambient mass spectrometry allows the direct characterization of raw samples at molecular levels under an open atmosphere[1]. Due to the unparalleled sensitivity and specificity, ambient mass spectrometry has been increasingly considered for wide applications in food science and industry. In virtually any type of food samples could be directly analyzed using mass spectrometry powered by one of the currently available ambient ionization techniques.

Typically, trace amounts of analytes on solid foods could be rapidly detected using either desorption electrospray ionization (DESI), direct analysis in real time (DART) or desorption atmospheric pressure chemical ionization (DAPCI)[1, 2]. Both volatile and non-volatile analytes could be detected by ambient ionization techniques for surface analysis. For more consistent results, heat, solvent extraction or gas liberation would be preferably used during the desorption/ionization process for detecting analytes of low volatility, particularly on viscous food surfaces (e.g., cheese, icy meat etc.). Alternatively, liquids and gaseous samples could be real time monitored using extractive electrospray ionization (EESI) or its derivates[3, 4].

Rapidly revealing the chemical information inside bulk foods is of paramount importance in food science and industry. Herein internal extractive electrospray ionization mass spectrometry (iEESI-MS)[5] was developed to probe the molecules inside a 3-D bulk food sample requiring no mashing/grinding the sample or matrix clean-up. To date iEESI-MS has been applied to the qualitative characterization of various biological samples (tissues, fruits, vegetables, etc.) and accurate quantitative analysis of 6 β-agonists in bulk meat samples, opening new possibilities in food science and industry.

In this talk, the principle of ambient mass spectrometry, typical instrumental setup and applications would be systematically reviewed, the advantages and shortages of ambient mass spectrometry for food analysis would be mentioned, and the impact of ambient mass spectrometry on food industry would be briefly discussed.


[1] Takáts, Z.; Wiseman, J. M.; Gologan, B.; Cooks, R. G. Science. 2004, 306, 471-473.
[2] Gross, J. H. Anal. Bioanal. Chem. 2014, 406, 63-80.
[3] Chen, H.; Yang, S.; Li, M.; Hu, B.; Li, J.; Wang, J. Angew. Chem. Int. Edit. 2010, 49, 3053-3056.
[4] Li, X.; Hu, B.; Ding, J.; Chen, H. Nat. Protoc. 2011, 6, 1010-1025.
[5] Zhang, H.; Chingin, K.; Zhu, L.; Chen, H. Anal. Chem. 2015, 87, 2878-2883.

Konstantin Chingin received his Ph.D at ETH Zurich (2010). He was a postdoc in the group of Prof. Richard N. Zare at Stanford University (2010-2011). He was a research fellow hosted by Prof. Roman Zubarev at Karolinska Institute, Stockholm (2011-2013). He is a full professor of Analytical Chemistry at East China University of Technology (2013-present). He published more than 40 peer-reviewed publications in SCI journals including PNAS, Angew. Chem., Anal. Chem., Chem. Commun., Mol. Cell. Prot., Sci. Rep., etc. His research is mainly focused on bioanalytical mass spectrometry.

Using Malt Extract for Yoghurt Production and Evaluating for Healthiness

Fatma Esra Gunes

Marmara University, Turkey

Malt extract is a source of nutrition, energy, vitamin B and sometimes it is used as a “tonic”. The purpose of this presentation is to show the effect of using malt extract in yogurt production and evaluate the end product in terms of health.

Malt extract is a very valuable nutrient since it contains easily digestible carbohydrates like dextrin and maltose, protein decaying products, enzymes and vitamins. Malted milk is used most widely. When malt and milk are combined during the milk production process, the fat globules are encapsulated and become protective against the oxidative stress.

In recent years, because of this suitability of malt and milk, they were started to be used in ice cream and cheese industry. Malt extract-added yogurt contains water-soluble fructans, fructo-oligosaccharides and some nutrients which exist in malt contents and are considered as prebiotics. The presence of these substances in food makes it a functional food.

By adding the malt extract, the appearance, flavor, texture and taste of the yogurt was optimized. Malt extract can be used in production of yogurt and it can be acceptable and supporting in the health perspective.

Keywords: Malt extract, yogurt production, prebiotic, functional food

Fatma Esra Gunes has Graduated from Hacettepe University, Nutrition and Dietetics Department in 1991, got her master degree from Department of Public Health, Faculty of Medicine, Selcuk University in 1994, her master thesis was “Determination of vitamin C levels in the blood and urine of young people who are smoker and nonsmoker” and got her PhD from Department of Food Hygene and Technology, Veterinary Faculty, Selcuk University in 2007, her doctorate thesis was “The use of malt extract in production of yogurt and cheese”. Interested in nutrition ecology, public health and epidemiology, nutritional epidemiology, food technology, clinical nutrition and nutritional medicine. Works since the December of 2008 at the Department of Nutrition and Dietetics of Health Sciences Faculty of the Marmara University, and is Associate Professor and the head of the department since 2016.

Do White Adipose Adipocytes Express Receptors for Gut Hormones?

Al-Naimi S, Ranganath L, Trayhurn P and Shenkin A

University of Suffolk, United Kingdom

Background: The gastrointestinal tract is the portal for the entry of dietary nutrients, and a number of intestinal hormones such as ghrelin, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are involved in their absorption and storage. A direct effect on adipose tissue is highly likely if specific receptors for these peptide hormones can be identified in the tissue. While specific receptors for GIP have been reported in adipocytes, studies have been inconclusive with regard to GLP-1 and nothing is known with respect to ghrelin.

Aim: To determine: (1) if the genes for the ghrelin, GLP-1 and GIP receptors are expressed in mouse (3T3-L1) and human (Simpson-Golabi-Behmel syndrome; SGBS) adipose tissue cell culture systems; (2) at what stage of adipocyte maturation, receptors for ghrelin, GLP-1 and GIP are expressed; (3) to assess whether receptors for GIP (GIPR), GLP-1 (GLP-1R) and ghrelin (ghrelinR) are expressed in each of the major mouse adipose tissue depots.

Outline methods: Species specific primers were designed and RT-PCR was performed on RNA extracted from mouse adipose tissue depots, and 3T3-L1 and SGBS cells. Expression of the genese for GIPR, GLP-1R and ghrelinR was examined.

Results: (1) GLP-1R, GIPR and ghrelinR mRNA was detected in several tissues, including four major white adipose tissue depots in mice. (2) 3T3-L1 and human SGBS adipocyte cell culture studies show that GIPR, GLP-1R and ghrelinR genes are expressed late post-differentiation.

Conclusion: GIP, GLP-1 and ghrelin receptors are expressed in the major mouse fat depots as well as mouse and human adipocyte cell culture systems. Hormonal signals from the intestine may influence adipocyte function.

Suha is an MB ChB, MSc, PhD qualified professional in the fields Chemical Pathology and Metabolic Medicine. With a broad based experience in both national and international environments. Previously she worked as a Clinical Research Fellow at the Biomedical Research Centre (BMRC), University of East Anglia (UEA). During her research project she established culture and manipulation of a new parasite to the labs as well as investigating the effects of this parasite on gut intestinal epithelia and as a result Suha has a numerous publications and received awards for her work. Moreover, whilst conducting research she got involved in facilitating both Problem Based Learning (PBL) and Inter Professional Learning (IPL) for students from the Medical School at UEA.

Antioxidant, Antidiarrhoeal & Anticancerous Effect of Nanocoated Food Packages (Glycyrrhiza glabra)

M. Sylvia Subapriya, M. Ramya and B. Nalini

Avinashilingam Institute for Home Science and Higher Education for Women, India

The commonly used polycarbonate bottles contain Bisphenol A (BPA). Exposure to BPA causes cancer and mimics the hormonal system in pregnant women and children. Only recently, the polycarbonate bottles are banned, in India. As an alternate to BPA, Polypropylene (PP) is used in baby bottles, drinking straws and microwavable wares. The major chemical migrants in PP namely, Octa decyl 3(3-5 di-tert-butyl-4-hydroxy phenyl) and propionate/ Iganox 1076 are known to cause cancer of liver, kidney and lungs in experimental animals. Hence the present study was conducted to synthesize a protective coating from four medicinal plants, of which Glycyrrhiza glabra was found to possess the highest antioxidant, antimicrobial and anticancer properties. The particle size, zeta potential and EDAX of nanoparticles from Glycyrrhiza glabrawere 34.7nm, -24.7mV and 2.60 percent of silver respectively. These properties along with the phytochemicals, augmented the chelating and antimicrobial potentialities of the plant extract. Thin layer chromatography of Glycyrrhiza glabra showed the presence of phenolic compounds and antioxidant activity of 520 μg. FTIR results revealed chlorohexidine salts peaks at 1157 cm-1, which indicate breaking up of lipopolysaccharide. Chlorohexidine is a cationic biocide that damages the outer membrane of gram negative bacteria. Secondly the peak 1442.72 cm-1 corresponds toC=O stretch which shows remarkable intensity changes, revealing better antimicrobial potentials of the encapsulated nanoparticle compared to the raw plant extract. The GC/MS identified the following compounds namely tetradecanoic acid, Z, Z 2,5 pentadecadiene-1-ol, cis-9,10 epoxy octadecan-1-ol, 9 octadecenal and hexadecanoic acid. Hence, Glycyrrhiza glabra nanoparticles were coated onto the feeding bottles and the foods stored in them were tested for their shelf-life for three hours. Nano coats of Glycyrrhiza glabra were also tested on pet bottles and ziplock covers and foods tested over 10 days. Milk stored in feeding bottles recorded a maximum microbial inhibition of 84 percentage and diluted lemon juice showed no microbial growth of S.dysenteriae. On 10th day, nanocoated zip lock covers and PET bottles showed a maximum microbial inhibition of 38, 63 and 83 percent and 57, 68 and 88 percentage against E.coli(MTCC 40), S.enterica (MTCC 3219) and S.dysenteriae (PSGIMS & R) respectively. The MTT assay of Glycyrrhiza glabra nanoparticles showed a maximum apoptosis of 50 and 48 percent against Hela and liver carcinoma cell lines respectively. The gel electrophoresis has led to a longer tail DNA damages with the Hela and carcinoma cell lines, compared to the control and marker. Therefore, nanocoats of Glycyrrhiza glabra could be used for effective protection against oxidative damage, diarrhea and cancer.

Dr. M. Sylvia Subapriya is a Professor in Department of Food Science and Nutrition having 24 years of teaching and research experience and is NSS Programme Coordinator, Avinashilingam University, Coimbatore. She has guided about 4 Ph.D. scholars and 60 post graduate students. To her credit, she has completed 3 national projects and Unicef SMART Survey. She has authored chapters in 2 books and published 12 international and 20 national level articles in refereed journals. Has also participated and presented papers in conferences at Kuantan and Kuala Lumpur, Malaysia. She is on the Editorial Board of the International Journal of Sports Sciences and Recreation, Malaysia Member in various committees and boards of national standards like Nutrition Society of India, Home Science Association of India, Indian Science Congress and Indian Dietetic Association.

Post-harvest Losses, Challenges of Scaling New Technologies & the Zerofly® Storage Bag

Allan Mortensen and Georgina V Bingham

Vestergaardʼs Food Security Organisation, Switzerland

Introduction: Food Security is an issue that will impact everyone by 2050 it is projected there will be a global crisis unless action is taken. Within the UN Sustainable Development Goals, Food Security is one of the key areas that will receive focus over the next 15 years to circumvent this crisis.

Method: The ZeroFly® Hermetic Storage Bag is a new technology developed to reduce post-harvest losses. It contains a safe insecticide, Deltamethrin, incorporated into a storage bag. The active ingredient is released on the surface of the material in a controlled and sustained manner ensuring food is continuously protected against insects. Insecticide residues found on grains stored for up to three years in ZeroFly® Storage Bag are below CODEX & EPA maximum residue levels. Additionally, a hermetic liner can be added to the bag to remove the need for fumigation of grains and seeds prior to entry into the bag.

Results/Conclusions: Laboratory and field studies in 11 countries show that the ZeroFly® Storage Bag can effectively control key stored product insects for up to 36 months. The presentation will explore the current scale-up efforts and strategies of distribution planned throughout Africa and Asia.

Allan Mortensen has a MA in International Business, Language and Culture from University of Southern Denmark. He joined Vestergaard in 2002 by relocating to Ghana opening up their West Africa Public Health Business by establishing offices in Ghana and Nigeria. With strong emphasis on business development Allan has opened businesses in Papua New Guinea, Australia, Ghana, Nigeria and Mongolia. Allan has developed and executed business models with in Public Health malaria prevention, Clean Water and today is the managing director of Vestergaardʼs food security organisation seeking to increase the real income of small and medium holder farmers in developing countries by introducing and taking to scale innovative tools to reduce postharvest losses.
Company Mission: Vestergaardʼs innovation in disease control textiles is fueled by our humanitarian entrepreneurship to create a healthier planet. Our corporate DNA is embedded in Humanitarian Entrepreneurship, meaning the ability and desire to combine doing business and doing good. The company produce and sell Lifestraw® (, PermaNet® ( and ZeroFly® (

Effect of Taxifolin with Lactic and Ascorbic Acids on Physicochemical and Microbiological Parameters of Dry-Cured Pork Sausages

Sonata Gustiene1, Gintare Zaborskiene2 and Anita Rokaityte1

1Lithuanian University of Health Sciences, Lithuania
2Kaunas University of Technology, Lithuania

The taxifolin (TXF) is recently rediscovered as a highly potential antioxidant with reported therapeutic properties. However, the data about possible protective properties of TXF and information about its stability is scarcein meat sausages. The aim of this work was to evaluate the antioxidant and antimicrobial activity of TXF when it is used together with ascorbic acid (AA), linalool, dipeptide carnosine(CAR), lactic acid (LA) and their mixtures indry-cured pork sausages during storage. Also, possible biochemical interactions among these bioactive substances on cell apoptosis and proliferation-related variables in the human cervical cancer HeLa cellshave been investigated. The activity of tested materials against lipid and protein oxidation, the grower of microorganisms and stability of TXF has been evaluated in dry-cured sausages after 0, 30, 60, 90 and 150 days of storage. The evaluation of TXF stability has been performed by UHPLC analysis. On the 150 day of storage the total amount of TXF was higher in sausages with TXF+LA (40% less) and with TXF+AA (54% less), comparingwith the 1st day of storage. Meanwhile, the retained amount of TXF in the samples without acids was 60% less, comparing with the initial amount. TXF antioxidant capacity (DPPH) was higher in the samples with LA or AA and effectively inhibited the processes of lipid peroxidation as well as slowed down the rate of lipolysis. Moreover, TXF mixtures with acids reduced the number of total aerobic bacteria, mold and yeast during storage of sausages. The treatment of combination of the three compounds (TXF, CAR and AA or LA) on HeLa cells (by MTT method) resulted in a significantly lower percentage of live cells number than treatmentonly with individual substances in different concentrations (P<0.05). The outcome of this study can help develop new meat products with better nutritional quality and beneficial health properties.

Sonata Gustiene was born in Lithuania August 17, 1985. In 2004, she finished a secondary school and entered the Lithuanian University of Health Sciences, Veterinary Academy, veterinary food safety speciality. In 2008, she got a Bachelorʼs degree in Public Health and continued her studies successfully for the Masterʼs degree. In 2010, she got a Masterʼs degree in Public Health. After the university she started working as a quality manager in the meat processing company. In 2016, she decided to improve her knowledge in doctoral studies at the Lithuanian University of Health Sciences, Veterinary Academy.
The working group she belong to has experience in investigation of food chemical composition and in evaluation of antioxidant influence on quality parameters in meat products as well as in creation of new analysis methods and development of functional meat products.
The foreseen dissertation topic: The Development and Application of Bio-preservation Tools Ensuring the Safety and Quality of Meat Products.

Effect of Supplementation Probiotic Curd (Indian Dahi) in the Prevention of Pre Diabetes to Diabetes Mellites

P. Amruth Rao1, P. Ramulu2 and Kavanashri N.M1

1National Institute of Nutrition, India
2Osmania Medical College and Hospital, India

Introduction: Diabetes mellitus comprises a group of metabolic disorders that share the feature of hyperglycaemia. It is worldwide in distribution and the incidence of both type 1 and type 2 diabetes is rising dramatically. Globally, diabetes is one of the most common non-communicable diseases leading to mortally and morbidity in many developed countries. The increased risk has been attributed to the high prevalence of multiple atherosclerotic risk factors among diabetic patients. Cardiovascular disease is increased in individuals with type 1 or type 2 DM. In patients with Type-2 DM, cardiovascular disease (CVD) is the major cause of morbidity and mortality. In fact, diabetes is considered as a coronary equivalent, approximately 80% of all deaths and more than 75% of all hospitalization in patients with diabetes is due to CVD.

Objective: To find out the association of dietary pattern and CVD risk factors, among recently diagnosed as Pre-diabetes subjects.

Methodology: This is a hospital based cross-sectional and case-control study with newly diagnose Pre-diabetes, aged 20-50yrs, of both sexes. Out of 50 subjects screened only 16 subjects were recruited as pre diabetes based on their HbA1c values (DCCT-5.7 to 6.5%) from the outpatient Department of Osmania General Hospital Hyderabad, India The various biochemical parameters viz Fasting blood glucose, HbA1c (glycated Hb) Lipid profile, i.e., Total Cholesterol, and Triglycerides LDL, VLDL and HD Lcholesterol were analyzed using commercially available kits.

Results: Our results indicate that there were significant changes in the values of Fasting blood glucose, Total cholesterol, Triglycerides etc upon probiotic supplementation for a period of 30 days in the Pre-Diabetes subjects compared to non supplemented control group.

Conclusions: From this study we could conclude that the probiotic curd supplementation may prevent the progression of pre diabetic mellitus over to diabetes mellitus.

Dr. P. Amrutha Rao completed MBBS from Gandhi Medical College, Osmania University in 1985. He also completed post graduate diploma in Public Health (DPH) from NTR University of Health Sciences in 1990. He joined in the department of Food and Drug Toxicology Research Centre of NIN in 1993. His area of specialization are, Public Health, Nutrition, Clinical Toxicology, Food Safety, Lathyrism and Food Borne Disease Outbreaks. He also participated in Iodine Deficiency Disorders (IDD) survey in North-East India. He is member of Nutrition Society of India (NSI), Indian Public Health Association (IPHA) and Indian Dietetics Association (IDA). Presented Oral Paper Presentation in Banaras Hindu University, Varanasi on “Lathyrism in South Central India”. Presently he is in-charge of Nutrition Unit in Osmania General Hospital. He is also faculty member for MSc (Applied Nutrition) and taking the Students to the Nutrition Ward and explaining the Clinical Signs and Symptoms of Nutritional Deficiency Diseases.

Effect of Rice Grain Discoloration on Qualitative Characters

Muhammad Saifulla and H. B. Shivaleela

UAS, GKVK, India

Rice varietyViz., IET 7191 affected by grain discoloration was selected to study its qualitative characters. Discoloration was resulted due to infection by fungiViz., Bipolaris oryzae, Pyricularia oryzae, Fusarium moniliforme, curvularia lunata, Trichoconiella padwickii, Cladosporium, Apergillus, Phomasp, Rhynchosporium oryzae, Alternaria alternata, Nigrospora oryzae, Absidia sp. etc. Rice grains were grouped in to four categories based on extent of discoloration viz., less than 1 per cent, 1-25 per cent, 26-50 per cent and more than 50 per cent discoloration. Grains under each category were subjected to milling and polishing. After milling and polishing kernels were plated on moist blotters to know the effect of milling and polishing onseed mycoflora. Only fungi viz., Bipolaris oryzae, Fusariummoniliforme, curvularai lunata, Trichoconiella padwickii, Cladosporium, Apergillus, and Alternaria alternata were observed on rice kernels after milling and polishing. Kernels after milling and polishing under eachcategory of discoloration were subjected tophysico-chemical compositions. As the level of discoloration increased from less than 1 per cent to more than 50 per cent discoloration, 1000 kernel weight decreased from 20.0 to 16.4 per cent, moisture content from 6.5 to 5.0 mm, raw rice length from 6.15 to 6.0 mm, raw rice width from 2.50 to 2.32 mm, length-width ratio increased from 2.46 to 2.58. Amylose content decreased from 24.0 to 9.0 per cent, total nitrogen content from 1.46 to 0.82 per cent, crude protein content from 9.12 5.16 per cent, reducing sugars from 34.75 to11.01 u moles, non reducing sugars increased from 0.26 to 93.95 u moles and total sugars from 35.01 to 104.76 u moles per gram of dry weight of rice kernels, while alkali value was not affected by discoloration.

Muhammad Saifulla worked as an Assistant Professor in UAS, GKVK, University of Agricultural Sciences, Bangalore, India. As an Associate Professor from 1995-2003, As Professor from 2003-2015 University of Agricultural Sciences, Bangalore, India.
Awards & Fellowships:
Member of “Rice blast working group on host resistance and pathogen population virulence. IRRI, Philippines.
Distinguished Leadership Award for having worked on the “Identification blast resistant rice genotypes and fungicides” The American Biographical Institute, Raleigh, California.
Fellow of Phytopathological Society of India. New Delhi.
Fellow of Mycology and Plant Pathology, Udaipur, India.
Honorary Appointment to “The Research Board of Advisors 1999” by the AmericanBiographical Institute.

Volatome Analysis for the Quality Determination in Virgin Olive Oil

Elettra Marone2, Mancuso S1, Fiorino P1 and Taiti C1

1Universita di Firenze, Italy
2Universita di Teramo, Italy

The quality of commercial virgin olive oils is guaranteed by chemical and organoleptic (Panel Test, PT) analyses.

The aim of this research was to transform VOCs perceived by the human olfactory sense in a meaningful spectra profile useful to distinguish oil trade classes (EVOO/ Not EVOO), and to grade EVOO, based on the intensity of the main positive (fruity) attribute using a fast and not expensive VOCs test, also useful to promote at consumerʼs level the sense of quality by smell and lack of defective odors.

Extensive surveys were carried out on 150 olive oil samples from different countries, processed as monocultivar or blend, acquired to producers or in the stores. Samples were analyzed by a PTR-ToF-MS as its high resolution coupled to a rapid screening power of samples, easy to handle and without any manipulation, while a PT recognized as EVOO 68/150 samples, characterized by different “fruity” flavor intensity.

A principal component analysis (PCA) of the whole PTR-ToF-MS data clustered the samples in two main groups, including the 68 samples classified by the PT as EVOO, without any defective odor, and the 82 classified as not EVOO, respectively, with only a few partially overlapped.

A PLS-DA model correctly classified EVOO/Not EVOO spectra as resulted by PT, confirming the high confidence level (95%) in utilizing analytical spectral data in helping PT.

A Fuzzy Clustering applied to the spectral data confirmed the 3 group of EVOO recognized by the Panelists, allowing to grade the samples by “fruity intensity” (1-3 = light, 4-6 = medium, 7-9 = intense).

In conclusion, throughout adequate DB the high quality of each oil sample can be predicted in any step of commercial chain of virgin olive oils processing, by a rapid and convenient way, so to define and preserve the high quality products.

Elettra Marone completed her Ph.D. in Productivity of crops. Researcher (Arboriculture), Faculty of Biosciences and Technologies for Agriculture, Food and Environment, University of Teramo, Italy. Current her teaching: Crop Production for the Agro-food Chain, Master of Science in Food Science & Technology (English). Academician, Accademia Nazionale dellʼOlivo e dellʼOlio. She is a Member of : ISHS, SOI, ACS, National Board for Olive Oil Tasters, Florence. Current research: Quality of temperate and tropical fruits for trade and consumers. Genetic characterization and VOCs in Coffea. Environment/ olive productivity, olive oil traceability and quality: VOCs biosynthesis and evolution, flavors/off-flavors. Oleuropein in olive cultivar leaves. Olive pomace for quality and healthiness of milk/dairy products.

Nutritious and Climate Smart Small Millets for Mitigating the Challenges of Malnutrition and Diabetes

M. Shivamurthy

University of Agricultural Sciences, India

Small millets have both preventive and curative properties good for addressing constipation, heart problems and type 2 diabetes suitable for preventing obesity. Small millets include, finger millet (Eleusinecoracana), Kodo millet (Paspalumscrobiculatum), little millet (Panicumsumatrense), foxtail millet (Setaria italic), proso millet (Panicummiliaceum), and barnyard millet (Echinochloacolona) and each one of them has special nutritional benefits. Small millets, perform well in marginal environments having superior nutritional properties, including high micronutrient and dietary fibre content, and low glycemic index. Despite their superior nutritional qualities and climate resilience, cultivation of small millets in India declined from 7.22 million hectares to 2.29 million hectares between 1961 and 2009, this decline in production and consumption of small millets mainly due to limited productivity, high drudgery involved in their processing, negative perceptions as a food for the poor. Through context-specific production technologies, creating decentralized processing infrastructure and local market development it is possible to bring back small millets to farms and food baskets on a wider scale. Among the children under the age of five years in India, 48 percent have stunted growth and 43 percent are underweight, likewise chronic and non-communicable diseases like Type-II diabetes and impaired glucose tolerance are on the increase. The primary factors responsible for the steep decline in the production and consumption of small millets in India are; Low productivity, high labour intensity, drudgery of agricultural operations and lack of attractive farm gate prices. Promoting small millets as cropping systems, support for timely availability of quality seed, Large scale capacity building to farmers on location specific improved production practices, creating post-harvest infrastructure and marketing initiatives are some of the strategies for enhancing production. Besides, therapeutic products targeting elites need intensive efforts for quality maintenance, consumption promotion through public support and supplying ready to cook small millet products will promote small millets in the baskets of urban elites are discussed in this paper.

Murudaiah Shivamurthy is A Visiting Scholar IB University of Ghent, Belgium and Humboldt State University, Berlin, Germany. At present he is Professor & Head of Agricultural Extension, College of Agriculture, UAS, GKVK, Bangalore – 560 065, India.
He worked as professor of Agricultural Extension, University of Agricultural Sciences, Bangalore from 2003 to 2016, Associate Professor of Agricultural Extension, University of Agricultural Sciences, Bangalore from 1995 to 2002, Assistant Professor of Agricultural Extension, University of Agricultural Sciences, Bangalore from 1991 to 1995.
Research articles 92
Articles in National and international conferences 108
Research note 04
Book chapters 04
Ph.D. students guided 9
M.Sc. Students Guided 24.

Association of Seed Mycoflora and their Management in Mungbean

Muhammad Saifulla and Devamani B. D

University of Agricultural Sciences, India

Mungbean is the third most important pulse crop in India. Among the fungal diseases, powdery mildew, anthracnose, Cercospora, web blight and dry root rot are the most prevalent. Apart from diseases some fungi are also causing qualitative and quantitative loss in storage. The study was conducted to know the seed mycoflora associated with mungbean and their management. Seeds samples collected from different districts were plated on moist blotters. Twelve fungi belonging to ten genera viz., Aspergillus niger, Alternaria alternata, Aspergillus flavus, A. candidus, Penicillium notatum, Rhizopus stolonifer, Cladosporiumsp., Fusarium oxysporum, Mucorsp., Curvularia lunata, Macrophomina phaseolina and Chaetomiumglobosum were observed from seed samples. Management of seed mycoflora was studied by different seed treatment methods viz., physical, chemical and biological methods. Seeds treated with hot water at 45 °C for 30 min and 50 °C for 20, 25 and 30 min reduced seed mycoflora. Seed treatment with dry heat treatment at 50 °C for one hour effectively reduced the seed mycoflora. Seed exposed to solar heat treatment between 12-2 pm effectively reduced the seed mycoflora. Seed treatment with captan recorded the maximum inhibition of seed mycoflora (78.68%) followed by carbendazim (68.30 %), thiram (65.57 %), chlorotholonil (62.29 %) and vitavax power (62.84 %) at the rate of 4 g kg-1 of seed. T. harzianumseed treatment at the rate of 8 g kg-1 of seed inhibited seed mycoflora up to 69.63 per cent followed by P. fluorescens (66.49 %), T. viride (64.39 %) and B. subtilis (63.79 %).

Muhammad Saifulla worked as an Assistant Professor in UAS, GKVK, University of Agricultural Sciences, Bangalore, India. As an Associate Professor from 1995-2003, As Professor from 2003-2015 University of Agricultural Sciences, Bangalore, India.
Awards & Fellowships:
Member of “Rice blast working group on host resistance and pathogen population virulence. IRRI, Philippines.
Distinguished Leadership Award for having worked on the “Identification blast resistant rice genotypes and fungicides” The American Biographical Institute, Raleigh, California.
Fellow of Phytopathological Society of India. New Delhi.
Fellow of Mycology and Plant Pathology, Udaipur, India.
Honorary Appointment to “The Research Board of Advisors 1999” by the AmericanBiographical Institute.

Exploring the Role of Date Pits Powder in Different Food Applications

Shahzad Hussain, Mohammed Saleh Alamri, Abdellatif Abdelhakim Mohamed, Akram. A. Abdo Qasem and Mohamed Abdrabo Ibraheem

King Saud University, Saudi Arabia

Dates (Phoenixdactylifera(L.) are extensively produced and consumed in Middle East. Dates flesh is consumed as a food while pits are thrown as waste or used as animal feed. Date pits comprise of 10-15% of total fruit weight. Date pits are rich source of dietary fiber (52%). Pits can be used in different types of food products to improve their dietary fiber contents. Bread loaves were prepared with acceptable sensory and rheological dough properties when date pits powder was replaced with wheat flour up to 12% level. The pasting properties of flour blends were also affected in the presence of date pits. Date pits pudding was also prepared and compared with commercially available chocolate pudding. Acceptable sensory and rheological attributes were observed in high fiber date pits pudding samples. Hardness and spread factor of cookies also increased in the presence of date pits powder. It can be concluded that date pits can be used as a cheap source of dietary fiber in different products without promising their quality attributes. The presence of good quality oil in date seed powder can also enhance the nutritional value of these food products.

Dr. Shahzad is holding a Ph.D degree in Food Science and Technology from National Institute of Food Science and technology, University of Agriculture Faisalabad, Pakistan. He has started my career as a faculty on Jan 2009 from his parent institute and currently working ad Associate Professor in the department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. Dr Shahzad has published more than 70 publications in the area of food science and nutrition and also presented his research at several international conferences. Currently, he is the part of 3 ongoing projects and teaching several courses related to safety, nutrition, composition, chemistry and analysis of foods. He is also managing the food rheology, baking, cereal, starch and thermal analysis labs from last 7 years.

Abscisic Acid (Aba) Andmethyl Jasmonate (Meja) Applications Increases Cracking Tolerance and Fruit Quality on Sweet Cherry Fruit (Cv. Bing)

Camilo Gutiérrez1 and Cristian Balbontin2

1University of BioBio, Chile
2Institute for Agriculture Research, Chile

Rain induced fruit cracking is an important problem in sweet cherry industry, causing loss up the 90%. Recent studies have suggested that a primary cause of fruit cracking could be the increase in fruit surface area during fruit development in the absence of deposition of cuticle membrane (CM) deposition. Abscisic Acid (ABA) and Methyl Jasmonate (MeJA) are phytohormones associated with stress tolerance, cuticle wax biosynthesis and fruit ripening. The objective of this research was to evaluate the effects of exogenous ABA plus MeJA applications on several quality parameters of sweet cherry fruits (cv. Bing). The application of theses hormones at different fruit development stages increased differentially the fruit cracking tolerance and fruit color at the ripening stage, affecting the soluble solids content, malic acid concentration and fruit cuticle resistance.

Acknowledgement: This work was supported by the FONDECYT 1150764 project and doctoral scholarship CONICYT.

Camilo Gutiérrez-Jara is a food engineer (Universidad delBío-bío, Chillán, Chile, 2013). Actually, he is a candidate to food engineering doctor (Universidad delBío-bío, Chillán, Chile, 2015). In this doctorate, his thesis is “Application of phytohormones and emulsified coatings to improve cracking tolerance of sweet cherry fruits”. The first thesis approach considers the application of phytohormones abscisic acid and methyl jasmonate(together and separately) in sweet cherry fruits. The second approach focus in the application of nano-emulsified edible coating alginate-based and soybean oil, in sweet cherry fruits. He is currently beginning to develop the second approach with the Dr. Ricardo Villalobos-Carvajal direction.

Effect of Selected Processing and Modification Methods on Quality of Cassava and its Starch

Adewale Olusegun Omolola and Patrick Francis Kapila

University of Venda, South Africa

The effect of processing on the quality properties of cassava and its starch was reviewed. Cassava (Manihot esculenta Crantz) is a broadly cultivated root crop and mostly consumed in developing nations. Cassava can be processed into flour, chips and starch for subsequent use in food production. Fermentation, boiling, drying, steaming, baking, blanching, frying and parboiling are some of the methods of processing cassava roots. These processes lead to a reduction in its cyanide content and other effects on cassava quality. The use of native cassava starch in food industry applications cannot be over emphasized; however, a major factor militating against its use is the finite imbalance in its structure and properties. As a result of this, native starch from cassava requires some form of modification to enhance its quality in terms of structure and functionality.

Keywords: Cassava; cyanide; quality indices; cassava processing; phytochemicals; anti-nutrients; fermentation; fortification

Adewale Olusegun Omolola is a postdoctoral fellow in the Department of Agricultural and Rural Engineering, University of Venda, South Africa. His research interest includes processing and preservation of food crops, food processing optimization, microstructure of dehydrated food crops, modeling and optimization of food processing operations.

Detection and Genotyping of Campylobacter Species in Broilers Meat

Seyed Ali Ghorashi1, Banowary B1, Connolly J1,2, Chenu J3, Groves P4, Ayton M1,2, Raidal S1,2 and Dang V T1,2

1Charles Sturt University, Australia
2Graham Centre for Agricultural Innovation Australia
3Birling Avian Laboratories, Australia
4The University of Sydney, Australia

Campylobacter is a gram-negative bacteria with at least 16 different species which some of these species are important in foodborne disease in human. Campylobacter is the most important cause of human bacterial gastroenteritis particularly in developed countries. Campylobacter coli (C. coli) and C. jejuni are the two most common species that are reported from enteric infections in Australia and usually are associated with poultry meat as contamination of carcasses may occur at processing plants. In this study, a rapid molecular diagnostic and genotyping method was developed to detect and differentiate C. coli and C. jejuni in broilers carcasses. The test was evaluated further by investigating additional human samples. The sensitivity and specificity of the test was 100% and 92%, respectively.

Keywords: Campylobacter, genotyping, poultry meat

Dr. Ali Ghorashi is a senior lecturer in Animal production and Health at the Charles Sturt University, Australia. He received his DVM degree from Tehran University and a Ph.D. from James Cook University in Australia. He worked at National Institute of Genetic Engineering and Biotechnology and moved to Melbourne University as a research fellow before joining Charles Sturt University. Dr. Ghorashi is a member of editorial board of five international scientific journals as well as professional organisations. His research interests are molecular diagnosis and genotyping of veterinary pathogens and molecular epidemiology of infectious diseases.

Study on Chemical Composition of centaureakarduchorumboiss. Species from Endemic Plants of Eastern Anatolia/Turkey

Ruveyde Tuncturk and Murat Tuncturk

Van Yuzuncu Yıl University, Van-Turkey

CentaureakarduchorumBoiss. Belonged to the Asteraceae (Compositae) which is represented by 1100 genera and about 25000 species in the world. It has a total 1209 species in the flora of Turkey. Interms of the number of species, Asteracea family is the richest family in our country. The genetic origin of Centaurea genus is turkey which this genus is quite common. This plantused as digestion, diuretic, antipyretic, antidiabetic, anti-inflammatory and antibacterial. Centaureakarduchorum Boiss species spread naturally in the Van lake in the Eastern Anatolia Region of Turkey and is a perennial endemic species. The leaves of are consumed as herbal tea by the local people for the treatment of diabetes.

In this study, the nutritional values and mineral compositions of CentaureakarduchorumBoiss which grown as wildplants in Van region of Eastern Anatolia, Turkey were examined. The chemical character sestimated included as N, Na, Mg, K, Ca, P, S, Mn, Fe, Cu, Zn, Cr, Cd, Co as well as total ash, crude protein, crude fiber and pH.

A result of research, the ash content was obtained as 5.93 %, nitrogen content 1.75 %, crude protein content 10.93 %, pH 6.66 and crude fiber 32.58 %. Also, the detected values were as follow: Potassium content 5.57 g/ phosphorus 1.18 g/kg, magnesium 5.71 g/kg, sulphur 1.12 g/kg, manganese 79.56 mg/kg, zinc 15.18 mg/kg ve iron 583.56 mg/kg.

Keywords: Anatolia, mineral elements, CentaureakarduchorumBoiss.

Ruveyde Tuncturk Graduated from Department of Field Crops, Faculty of Agricultural of YuzuncuYil University in 1998, got her master degree from same depertmant and university in 2001, her master thesis was “The Effect of Different Nitrogen Doses and Within Rows on Yield and Character of Potato (Solanumtuberosum L.) In Van Ecological Conditions” and got her Phd from same department and university in 2010, her doctorate thesis was “The Effects of Various Fertilizer Sources, Sowing Dates and Bacteria Inoculation on The Yield and Yield Components of Fenugreek (Trigonellafoenum-graecumL.) In Van Ecological Conditions”. Interested in various industrial plants (oil plants, starch and sugar plants, fiber plants), seed technology and physiology. Works since the December of 2001 at the Department of Field Crops, Faculty of Agricultural of YuzuncuYil University and now she is Associate Professor since 2013.

Effects of Post harvest UV-C and Hot Water Applications on Fruit Quality and Cold Storage in Ziraat 900 Sweet Cherry Cultivar

Cavusoglu Seyda, Tekin Onur, Bahar Aşkın, Ozrenk Koray and Nevzat Durmaz

YuzuncuYıl University, Van-Turkey

In this study, the aim is to determine the storage condition of Ziraat 900 sweet cherry cultivar in province of Mersin in Turkey and to find out the effectiveness of treating the UV-C, Hot Water and the combination of both in protecting the fruit quality during storage in MAP (modified atmosphere packaging). For this aim, clusters of control group were not subjected to any application in the postharvest period, while clusters of treatment group were performed with VilberLourmat UV-C lamp glimmered 254 nm wavelength from 100 (0.25 kJ / m2) cm distance for 4 minutes in the sterile cabinet without any fumigation. Hot water application was also performed by maintaining of clusters in the water bath at 50 °C for 1 minutes. After treatment, by covering of all clusters (in the control and treatment groups) with stretch film with packing containers called modified atmosphere packaging, all clusters were stored at 0 ± 1 °C and 90 ± 5% relative humidity conditions in the cold storage room. For this purpose, cherry fruits was kept for 20 days in 0°C cold storage. Weight loss and color (L*, a*, b*, Chroma, and Hue), SSC (soluble solid content), titratable acidity, pH, total phenolics and total antioxidant changes that occurred postharvest storage were examined. As a result of this study, an increase in weight loss was detected during storage in all applications. As a result of study, it was concluded that cluster of Ziraat 900 sweet cherry cultivars subjected to hot water and UV-C can be stored for optimal 20 days by covering with stretch film.

Keywords: Sweet cherry, storage, UV-C, hot water application, modified atmosphere packaging

Seyda Cavusoglu has graduated from Department of Horticulture, Faculty of Agricultural of Van YuzuncuYil University in 1996, got her master degree from same department and university in 2000, her master thesis was “A research on the optimum harvest dates and storage of some pear varieties grown in Van province” and got her Phd from Department of Horticulture, Faculty of Agricultural of Ankara University in 2008, her doctorate thesis was “ The effect of pre-harvest cytokinin application on post harvest physiology of cauliflower Brassica oleracea L. botrytis”. Interested with post harvest, controlled atmosphere, plant hormone, fruit and vegetable. Works since the December of 1997 at the Department of Horticulture, Faculty of Agricultural of Van YuzuncuYil University and now she is Assistant Professor since 2010.