Food Matrices Enriched with Horseradish Products and Their Bioavailability

Lolita Tomsone^*, Zanda Kruma, Ruta Galoburda, Kristine Majore, Sanita Sazonova and Ruta Kazemaka

Faculty of Food Technology, Latvia University of Life Sciences and Technologies, Latvia

Horseradish is a plant of the cruciferous family rich in bioactive compounds. By enriching various food matrices with such plant products, it is possible to provide benefits to final consumers. The aim of the study was to investigate the effect of horseradish products on the physicochemical properties of different food matrices, as well as to evaluate the in vitro bioavailability. Horseradish products (microencapsulated leaf and root juice, leaf and root pomace) were added to food matrices such as pasta, butter cookies, fresh pork chops and minced pork. Food matrices without added horseradish products were used as controls. The total phenol content (TPC) and antioxidant activity was determined before and after treatment in an in vitro model of the human digestive tract, and calculated their bioavailability index (PAC). Food matrices enriched with horseradish products had significantly higher TPC and antioxidant activity than control samples. In previous studies horseradish leaves had a higher content of bioactive compounds than horseradish roots. Consequently, added horseradish leaf products showed higher efficiency than root products. The added horseradish products increased the TPC bioavailability index (PAC) in the analyzed food matrices. Horseradish products have a higher potential of functional ingredients and the results of the study show a significant benefit of enriching the analyzed food matrices with phenolic compounds. The TPC bioavailability index also confirmed high bioavailability. Further research is needed to optimizē the formulations of functional food matrices, making them more attractive to final consumers, as well as examining the health effects of added bioactive compounds.

Acknowledgments: This research was funded by European Regional Development Fund Post-doctoral Research Support Program (project No.1.1.1.2/16/I/001), grant number 1.1.1.2./VIAA/1/16/187.

Biography:
Dr.sc.ing., Mg.oec. L.Tomsone has been working in science since 2010 and has participated in several scientific projects-research as a researcher and leading researcher:
*ERDF Post-doctoral Research Support Program (project No.1.1.1.2/16/I/001), grant number 1.1.1.2./VIAA/1/16/187;
*State Research Programme „Sustainable use of local agricultural resources for development of high nutritive value food products (Food)” (VPP2014-2017)”;
*Project No. K24 (2012.–2013.) within the framework of the cooperation programme between Latvia and France in science and technology development areas “OSMOZE”.

Identification of Gastrointestinal Digestion Stable Antihypertensive Fish Peptides from Atlantic Mackerel (Scomberscombrus)

SoheilaAbachi Hokmabadinazhad^1*, Jacinthe Thibodeau², Ismail Fliss³, André Marette⁴, Laurent Bazinet⁵ and Lucie Beaulieu⁶

¹Institute of Nutrition and Functional Foods, Université Laval, Canada
²Laboratory of Food Processing and ElectroMembrane Processes, Université Laval, Canada
³Institute of Nutrition and Functional Foods, Université Laval, Canada
⁴Department of Medicine, Cardiology Axis of the Quebec Heart and Lung Institute, Canada
⁵Laboratory of Food Processing and ElectroMembrane Processes, Université Laval, Canada
⁶Institute of Nutrition and Functional Foods, Université Laval, Canada

High blood pressure has been recognized as one of the main risk factors of cardiometabolic syndrome. In addition, there is a link between the occurrence of insulin resistance and hypertension consequently initiating type-2 diabetes. Hypertension is medicated by various classes of synthetic drugs; however, adverse effects have repeatedly been reported. To avoid and reduce side effects, natural alternatives such as marine biopeptides have been shown effective in the treatment and prevention of hypertension and its associated complications. The aim of present work was to fractionate and identify antihypertensive peptides by means of measuring the angiotensin-converting enzyme (ACE) inhibition capacity of fractions combined with UPLC-MS identification. Fractions were produced using different techniques of solid phase extraction (SPE), pressure-driven ultrafiltration (UF) and electrodialysis with UF membrane (EDUF). According to our resultsthe hydrophobic fraction of SPE with the IC50 value of 5 µg of protein was the most potent anti-ACE product. Findings of the study suggest that separation technique plays an important role in the isolation efficiency of antihypertensive biopeptides since the IC50 values of EDUF and UF fractions were higher and or insignificantly different from the IC50 value of the hydrolysate, 8.8 ug of protein. Importantly, the hydrophobic fractionʼs activity was retained through in-vitro gastrointestinal digestion system. In conclusion, polarity regardless of other properties, was the most important factor for anti-ACE activity of the fraction. In addition, the presence of leucine at either of the extremities and or leucine rich motifs could well explain the hypotensive effect of the active fractions.

Biography:
Soheila Abachi studied the antimicrobial activity of Atlantic Canada fruits/berries against Streptococcus pyogenes, its adhesion, biofilm formation and eradication at the Dalhousie University, Canada and graduated as M.Sc. in 2016. She right after joined the research group of professor Dr. Lucie Beaulieu at the Institute of Nutrition and Functional Foods, Université Laval, Canada to work on the marine biopeptides. She has several publications in the field of plant bioactives and will soon publish a number of research and review articles in the field of marine biopeptides.