Konkuk University School of Medicine, South Korea
Ultraviolet A (UVA) can penetrate further than the skin dermis and produce reactive oxygen species (ROS) in the dermal layer. ROS leads to changes of gene expression, inflammatory reactions in dermal tissue and photo aging. Therefore, strategies to protect the skin from UVA are constantly being studied. 23-Hydroxytormentic acid (23-HTA) has been proven to exhibit several free radical scavenging effects. The objectives of this study were to investigate the in vitro effect of 23-HTA on normal human dermal fibroblasts (NHDF) irradiated with UVA. The NHDFs derived from human skin cells were cultured in the medium diluted with 23-HTA and L-ascorbic acid. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertazolium bromide (MTT) assay was performed to investigate effect of 23-HTA on cell viability. Scavenging activities of 23-HTA against diphenylpicrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and hydrogen peroxide in vitro were evaluated. Quantitative real time PCR (qRT-PCR) analysis was used for the expression levels of antioxidant enzymes and pro-inflammatory cytokines genes. 23-HTA had the concentration-dependent radical inhibitory effects and exhibited protective effects against UVA-induced stress in NHDFs. In addition, decreased mRNA expression of TNF-α, IL-1 and IL-6 suggested that 23-HTA attenuated the UVA-induced inflammatory response by decreasing the expression of the proinflammatory cytokines. This study demonstrated that 23-HTA exerts a protective effect on human dermal fibroblast against oxidative stress induced by UVA.
Young Ah Cho is currently working for Konkuk university medical center in South Korea. She received the Bachelor’s degree in life science from the Hanyang University and received M.D. in Konkuk University, College of Medicine, South Korea. Her current research interests include proinflammatory cytokines and autoimmune disease in dermatology.
Konkuk University School of Medicine, South Korea
Alopecia, characterized by the loss of hair density or hair thinning, affects the quality of life in millions of people worldwide. Thus, many studies have been conducted to elucidate the mechanisms of hair loss and identify treatment candidates. The falconoid silibinin, extracted from Silybum marianum, has antioxidant properties and protective effects against skin damages. However, there has been no studies on the effects of silibinin on hair growth. The purpose of this study is to investigate the effect of silibinin on hair induction in human dermal papilla (DP) cells. Human hair follicle DP cells were three-dimensionally (3D) cultured and seeded on a 96-wellmicroplate. After the formation of the unified spheres, they were treated with silibinin for 48 hours. The size of the spheres were measured with a phase-contrast microscopy. Luciferase reporter assay was used to analyze the activation of the Wnt/β-catenin signaling pathways and western blotting was performed to assess the levels of serine/threonine kinase (AKT) protein. The upregulation of DP signature genes, including ALPL, VCAN, BMP2 and FGF7 was investigated through quantitative real time-PCR (qRT-PCR) analysis. Silibinin increased the diameter of 3D DP spheroids and induced AKT activation. Along with this, silibinin-treated DP spheroids significantly indicated activation of the Wnt/ - catenin signaling pathway. This study also revealed that DP signature genes related with hair induction were upregulated by silibinin in 3D DP spheroids. In conclusion, flavonoid silibinin increases hair proliferation through activation of Akt and Wnt/β-catenin signaling. For the treatment of alopecia, silibinin may have therapeutic potential.
Ji Su Lee is currently working for Konkuk university medical center in South Korea. Lee received the Bachelor’s degree in Interior Design from the Yonsei University and received M.D. in Konkuk University, College of Medicine, South Korea. Her current research interests include antioxidant therapy and alopecia in dermatology.
University of Lome, Togo, West Africa
Microorganisms have been recognized as a main target for medical treatment of acne because induced acne by several physiopathological mechanisms. Antibiotics are often prescribed in acne treatment. These antibiotics have sides effects and in recent year Antibiotic resistant acne-developing microorganism strains has been increasing. The use of plant in the treatment of many pathogenic microorganism is promising. Aloe buettneri, Mitracarpusscaber, Hannoaundulatawere three plants resources used in Togolese cosmetopeae and traditional medicine by treating dermatosis. This study aimed to assess in vitro antimicrobial activities of these three selected plants resources on acne-developing microorganism and other organisms isolated from acne lesions. The microbial germs used consisted of six reference strains: Propionibacterium acnes ATCC 6919, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603and Candida albicans ATCC 35659.The results showed microorganisms strains tested except Candida albicans and E. Coli were susceptible to plant resources extract at 250mg/ mlby the agar well diffusion method with inhibition diameters ranging from 12±2 to 18± 1 mm. The MICs values were in the range of 15,625 to 62,5mg/ml by macrodilution method and MCB between 31, 25- 125 mg/ml. The three plant resources are potential natural and effective alternative for treating in vitro acne and overcoming the emerging antibiotic resistance. Keywords: Acne, Antimicrobial activity, Cutibacterium acne, Aloe buettneri, Mitracarpusscaber, Hannoaundulata
Metowogo Kossi is currently working as Lecture and Researcher at University of Lome, Faculte des Sciences Laboratoire de Physiologie- Pharmacologie. He has completed his Ph.D. in Physiology, Pharmacology from Universite de Lome, Togo. His main area of interest focuses on Physiology and Pharmacology. His area of expertise includes Natural Substance, Pharmacology, Toxicology, Gastric Ulcers, Asthma, Wound Healing and Phytochemical Screening. He has 7 publications in journals as author/co-author.