Madridge Journal of Pharmaceutical Research

ISSN: 2638-1591

2nd International Conference on Pharma & Nutrition, Health and Aging

August 1-2, 2019, Valencia, Spain
Poster Session Abstracts
DOI: 2638-1591.a3.007

Cur2004-8, A Synthetic Curcumin Derivative, Extends Lifespan and Modulates Age-Related Physiological Changes in Caenorhabditis elegans

Sang-Kyu Park1*, Sung-A Kim1, Bo-Kyoung Kim1, Sun-Mi Baek1 and Eun-Young Lee2

1Department of Medical Biotechnology, Soonchunhyang University, Republic of Korea
2Department of Nephrology, School of Medicine, Soonchunhyang University, Republic of Korea

Curcumin is a compound found in yellow indicant curry and known to have various biological activities, including anti-oxidant, anti-inflammatory and anti-cancer activities. Cur2004-8 is a synthetic curcumin derivative having symmetrical bis-alkynyl pyridines and shows a strong anti-angiogenic activity. In the present study, we examined the effect of dietary supplementation with Cur2004-8 on response to environmental stresses and aging using Caenorhabditis elegans as a model system. Dietary intervention with Cur2004-8 significantly increased resistance to oxidative stress. Anti-oxidative-stress effect was greater than curcumin. However, response to heat stress or ultraviolet irradiation was not affected by Cur2004-8. Next, we examined effect of Cur2004-8 on aging. Cur2004-8 significantly extended both mean and maximum lifespan accompanying a shift in time-course distribution of progeny production. Age-related decline in motility was also delayed by supplementation with Cur2004-8. In addition, Cur2004-8 prevented amyloid-beta-induced toxicity in Alzheimerʼs disease model animals, which required a FOXO transcription factor DAF-16. Compared to curcumin, Cur2004-8 showed greater anti-oxidant and anti-aging effects. Dietary supplementation with Cur2004-8 also reversed increased mortality observed in worms treated with high-glucose-diet. These results suggest that Cur2004-8 a synthetic curcumin derivative harboring enhanced bioactivities and can be used for the development of novel anti-aging product.

Biography:
Sang-Kyu Park got his masterʼs degree in Biochemistry at Yonsei University in Korea. He got his Ph. D degree in Genetics at University of WisconsinMadion, USA in 2006. His thesis was about the identification of biomarkers of aging using transcriptional profiling. Then, he had worked as a Postdoc researcher at Institute for Behavioral Genetics at University of Colorado at Boulder and studied role of oxidative stress in aging. In 2010, he was employed as an Assistant Professor at Soonchunhyang University in Korea and has been studying the effect of anti-oxidants on aging and their underlying mechanisms using C. elegans.

Influence of Cyanobacteria Spirulina on Growth of Gut Microbiota

Gabriela Krausova1*, Bc. Katerina Karpiskova2, Ing. Jana Smolova1, Simona Lucakova3 and Tomas Branyik2

1Dairy Research Institute, Czech Republic
2University of Chemistry and Technology, Czech Republic
3Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Czech Republic

Algae and cyanobacteria are nowadays of great potential, especially for the food industry, where they are used as a food additive. Thanks to their nutritional value and beneficial effects on health, the genus Chlorella (microalgae) and the genus Spirulina (cyanobacteria) are commonly used as food supplements. Microalgae and Cyanobacteria represent a rich source of nutritionally valuable substances, including proteins, vitamins, pigments, antioxidants, unsaturated fatty acids and last but not least, oligo- and poly-saccharides, which can serve as an energy source for gut bacteria and thus serve as prebiotics. The aim of the study was to test the prebiotic effect of Spirulina biomass on bacteria from the genera Lactobacillus (5 strains) and Bifidobacterium (3 strains). The experiment was performed with both the commercially available Spirulina biomass and the laboratory-cultivated Spirulina. A positive prebiotic effect of laboratory-cultivated Spirulina was observed in two lactobacilli strains, in Lbc. animalis CCDM 382 and Lbc. acidophilus CCDM 151. In the rest of the strains tested, the prebiotic effect was not significant but there was no growth inhibition either. In the case of commercially available Spirulina, a positive prebiotic effect was observed in all strains tested (8), wherein, the growth of bacteria increased with the rising concentration of the biomass. Based on the results obtained, Spirulina can act as prebiotic by increasing the number of probiotic bacteria, while this ability is strain and substrate specific. It has been shown that there are differences in the prebiotic effect between the laboratory-cultivated and commercially available Spirulina. It can be assumed that the form and method of processing the biomass will play a role, the differences may also be due to different cultivation conditions and subsequent processing, particularly in the cell disintegration stage.

Biography:
Gabriela Krausová (born Kunová), graduated in 2006 at University of Veterinary Medicine in Košice, Slovakia, in 2014 finished her doctoral studies at the Czech University of Life Sciences in Prague, Czech Republic. Since 2008 she works at Dairy Research Institute in Prague as a researcher and since 2014 as the head of Department of Microbiology and Technology. She is the author or co-author of 11 papers indexed in the Web of Science database, 20 articles in reviewed journals, 2 certified methodologies, 1 patent and 10 utility models. Her topics of interest are food microbiology, functional foods, probiotics, prebiotics, food hygiene etc.

Oxidative Stress and Nutrition Profile in a Population of Patients with Breast Cancer in Western Algeria

Bensaber Hayette Senia1*, D. J. Bicout2, A. M. Bensnouci3, A. Comez4, Y. Chebloune5 and F. Z. El Kébir1

1Laboratory of Biology of Development and Differenciation (LBDD), Department of Biology, Faculty of Science, University of Oran, Algeria
2Laboratory of Team Environment Modeling and Prediction for the Heath of Populations (TIMC), Faculty of Medicine, France
3Laboratory of Pathology Regional Military Hospital and University of Oran (HMRUO), Algeria
4Laboratory of Anatomy and Pathological Cytology Hospital Gui De Chauliac, France
5Laboratory of Adaptation and Pathogenesis of Microorganisms (LAPMO), France

Breast cancer BC is the most common cancer in women worldwide, in particular in Algeria where it occupies the first place in term of incidence and mortality. The objective of this thesis is to estimate the risk factors of breast cancer among women in the West of Algeria. A case—control study of nutrition and breast cancer was conducted with 191 women of which 77 breast cancer cases and 114 control subjects to determine the relationships between leptin concentrations, lipid alterations, oxidant/antioxidant status, In vitro lipoprotein oxidizability and fatty acid composition in breast cancer. Glucose, insulin, leptin, lipids, fatty acids, markers of oxidant status hydroperoxides, carbonyl proteins, conjugated dienes and markers of antioxidant status vitamins A, C, E, erythrocyte activities of the enzymes superoxide dismutase SOD, catalase, glutathione peroxidase, glutathione reductase and the serum total antioxidant status ORAC were investigated in breast cancer patients and in control women. Our findings showed that insulin, leptin, triglyceride, cholesterol and LDL-C concentrations were increased in patients compared to controls. ORAC and vitamin C and E values were lower while plasma hydroperoxide, carbonyl proteins and conjugated dienes levels, SOD and glutathione peroxidase activities were higher than in controls. Fatty acid composition was altered with high saturated fatty acids and low polyunsaturated fatty acids. There were significant positive correlations between leptin concentrations and LDL-C, hydroperoxides, carbonyl proteins, SOD activity, conjugated dienes levels and oxidation rate and significant negative correlations between leptin and ORAC, lag time and polyunsaturated fatty acids in patients. The multivariate analysis showed the significant interaction between the markers of the antioxydantstatut, the lipid metabolism, the age of the menopause, the lifestyle and the genetic predisposition and breast cancer. In conclusion, this study suggested that breast cancer is associated with lipid alterations and enhanced oxidative stress linked to high leptin levels. The age of the menopause, the lifestyle and the genetic predisposition are predictive factors of BC.

Keywords: Breast cancer, Fatty acids, Leptin, Lipids, Lipoproteins, Oxidative stress.

Biography:
Bensaber Hayette Senia is a researcher and a lecturer class B who provides two modules in Immunopathology and Techniques of Genetics and Cancer in the Department of Applied Molecular Genetics at the Faculty of Science of Nature and Life University of Science and Technology of Oran Mohammed_Boudiaf USTOMB. She is a part of a research team specializing in embryogenesis and oncogenesis in a Laboratory of Developmental Biology and Differentiation LBDD. She has published several scientific articles, one of which is entitled Molecular Detection of Epstein Barr Virus in Women with Breast Cancer in West Algeria in the Journal of Cancer Therapy.

Investigating the Transdermal Delivery of Different Statins

Jeanetta du Plessis*, Suzanne Marais, Sumari Maree, Lissinda du Plessis and Minja Gerber

North-West University, South Africa

Statins are classified as 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase inhibitors and are the first-line of treatment for primary hyperlipidemia due to their ability to lower low density lipoprotein (LDL). After oral administration, statins may cause gastrointestinal side-effects and increasing the risk for hepatic toxicity. Hence, the aforementioned can be avoided by using another route of administration, i.e. transdermal delivery. During this study, female abdominal skin (Caucasian) was used, which were obtained after abdominoplasty (Ethics no: NWU-00111-17-A1).

The aim of the study was to investigate the transdermal delivery of the selected statins after being incorporated into different formulas. The formulas used during this study were nano-emulsions and nano-emulgels, which contained 2% of the selected statin and 8% apricot kernel oil as oil phase. After formulation, vertical Franz cells were used for the membrane release studies and skin diffusion studies. Tape stripping was performed after the 12h extraction of the receptor phase. All samples were analyzed by high performance liquid chromatography (HPLC). After the different formulas with the selected statins were compared, it was evident that the nano-emulsion containing rosuvastatin (NE1R) had the highest median flux value (413.650 μg/cm2.h) after the membrane release study, the nano-emulgel containing simvastatin (NEGS) delivered the highest median amount per area diffused (3.244 μg/cm2) after 12h transdermally, (NEGS) also had the highest median concentration (7.517μg/ml) in the stratum corneum-epidermis and the nano-emulsion containing atorvastatin (NE1A) delivered the highest median concentration (24.555 μg/ml) in the epidermis-dermis. Hence, all statins were delivered transdermally.

Biography:
Jeanetta du Plessis, Deputy Dean: Research and Innovation of the Faculty of Health Sciences and Professor of Pharmaceutics, is the author of about 100 research papers and 3 chapters in books. Since 1993, she supervised 18 PhD and 72 MSc degrees in her research program. The novelty of her research is reflected by an international patent, which was registered in nine countries. She is a member of the Academy of Science of South Africa (ASSAf) and the Organization for Women in Science for the Developing World (OWSD). She is also a member of the National Health Research Committee.

Approaches of Pharmacy Services Students in Vocational School of Health Services on Rational Drug Applications

Sultan Mehtap Buyuker1* and Odabasi G2

1Department of Forensic Pharmacology and Forensic Toxicology, Institute of Addiction and Forensic Science, Uskudar University, Turkey
2Uskudar University, Turkey

The aim of this study is to evaluate the knowledge and behaviors of the Pharmacy Services Program of Vocational School of health Services(VSHS) students about the rational drug use and to provide students with sufficient knowledge about rational drug use in the Pharmacy Services Program in the light of the data obtained from this study.

This cross-sectional study, conducted between 2017 October and 2018 March, was conducted on the first and second year students of a University VSHS Pharmacy Services Program. In this study, questionnaire was applied to 115 students to assess their knowledge and behaviour about the rational drug use. In the analysis of the data, the mean ± standard deviation and frequency were used, and the chi-square test was used for comparison between the groups.

The mean age of 115 students was 19.54 ± 1.79 years. Headache, cold, flu (64.6%) are the leading causes of drug use among the participants. Students were asked to recommend drugs and 30.7% of the participants stated that they recommended medication. The prominence of advertising in the drug was questioned and 58.8% of the participants stated that they did not use advertising products.

As a result of the research, it was seen that the authorities and responsibilities of pharmacy technicians should be emphasized more intensively, they did not prefer advertised drugs for treatment purposes and they should first consult with the physician and then the pharmacist in case of illness.

Keywords: Drugs, drug usage, pharmacy technician, rational drug use, students.

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