International Nanotechnology Conference & Expo
April 04-06, 2016 | Baltimore, USA
Preparation and characterization of chitosan-silver nanocomposite with anticandidal activity
Chungnam National University, Republic of Korea
Chitosan-silver nanocomposite (CAgNCs) was green synthesized without the adding any external chemical-reducing agents using low molecular weight chitosan (LMW-C) and silver nitrate. The synthesized nanocomposite was characterized by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), high-resolution transmission electron microscopy (HR-TEM) with selected area electron diffraction (SAED), Inductively coupled plasma–atomic emission spectrometer (ICP–AES), particle size distribution and zeta potential analyzer. The average size of LMW-C and CAgNCs were 1776 ± 23 nm and 240.1 ± 23.6 nm, respectively. The zeta potential of CAgNCs observed as + 41.1 mV. It was indicating positively charged polymeric layer on AgNPs surface. The AgNPs are deposited on chitosan matrix and the average AgNPs particle size lies between 5 nm- 50 nm. The Ag content of the CAgNCs was 0.696± 0.054% (w/w). The CAgNCs also proved to be an excellent antifungal agent against candida albicans. The minimum inhibitory concentration (MIC) values of CAgNCs and LMW-C for C. albicans were recorded as 50 and 100 µg/mL, whereas the minimum fungicidal concentration (MFC) values of CAgNCs and LMW-C for C. albicans were recorded as 150 and 400 µg/mL respectively. Propidiumiodide (PI) uptake results suggested that CAgNCs has affected to permeability of cell membrane of C. albicans Also, CAgNCs induced the higher level of reactive oxygen species (ROS) when compare to the LMW-C in concentration dependent manner. This report illustrates the eco-friendly approach for the reduction of silver using chitosan as a reducing agent, and its potential antifungal agent against C. albicans.
A Science Graduate holding a B.Sc special degree in Chemistry (University of Ruhuna, Sri Lanka) and M.Sc in Industrial Chemistry (University of Peradeniya, Sri Lanka). Now, I am working as graduate student following Master- PhD joint program at the College of Veterinary Medicine, Chunganm National University, and Republic of Korea. I have more than 5 yeses experience in nanotechnology and the author of two book (Sinhala language) and 8 scientific publication. Currently, I am working to develop biodegradable chitosan nano particle and chitosan metal (Silver and Gold) nanocomposite against pathogenic microbial.