International Translational and Regenerative Medicine Conference
April 25-27, 2018 | Rome, Italy
Development of a 3D Cell Culture Model Based on Biocompatible Polymeric Scaffolds Engineered with Human Mesenchymal Stromal Cells (Mscs) for Skin, Cartilage and Bone Regenerative Therapy
1Unit of Blood Diseases and Adult Bone Marrow Transplantation - Department of Clinical and Experimental Sciences - University of Brescia, Italy
2Materials Science and Technology - Department of Mechanical and Industrial Engineering - University of Brescia, Italy
3Laboratory of Manipulation and Cryopreservation of Stem Cells - Immunohaematology and Transfusion Medicine Service - ASST Spedali Civili di Brescia, Italy
4Section of Anatomy - Department of Clinical and Experimental Sciences, University of Brescia, Italy
5Chair of Biomedical Engineering - University of Glasgow, UK
6Electrical and Electronic Measurements - Department of Information Engineering - University of Brescia, Italy
7OU Orthopedics and Traumatology - ASST Spedali Civili di Brescia (Presidio of Gardone Val Trompia), Italy
8Division of Dermatology - Department of Clinical and Experimental Sciences - University of Brescia, Italy
9Odontostomatological Diseases - Department of Medical-Surgical Specialties, Radiological Sciences and Public Health - University of Brescia, Italy 10Diagnostic Imaging and Radiotherapy - Department of Medical-Surgical Specialties, Radiological Sciences and Public Health - University of Brescia, Italy
Regenerative medicine aims to restore normal function by repairing or replacing damaged cells and tissues in patients. The efficacy and the success of regenerative medicine depends on many factors including the manner by which the cells are organized in the new tissue that must be able to mimic the structure and function of the original one. Current treatments for articular cartilage damage, bone defects and skin lesions are quite challenging and they show limited repair and regeneration following injury.
This project aims at developing an innovative 3D cell culture model composed of Mesenchymal Stromal Cells (MSCs) and biocompatible, safe, resorbable, polymeric scaffolds for studying the mechanisms involved in tissue repair and treatment of chronic skin conditions, cartilage defects and bone lesions.
For this purpose, hydrogel-forming polymers scaffolds of natural origin were firstly characterized for their mechanical and morphological properties, stability and degradability. Secondly, MSCs were seeded on these scaffolds and optimal culture conditions for MSCs expansion were defined. Preliminary results suggest that hydrogel scaffolds seem to be an optimal model to host MSCs.
Currently, we are exploring the effects of scaffolds on modulating MSCs behaviour, in particular in the matter of how scaffolds can affect MSCs proliferation and differentiation. Moreover, efforts are being made to implement this 3D culture system with autonomous sensors in order to have an objective way to monitor tissue restoration.
This project will provide new useful insights to define novel innovative therapeutic approaches, thus making this research translatable within the clinical scenario.
Dr. Federica Re has obtained her Degree in Medical Biotechnology from 2010 to 2015 at the Department of Molecular and Translational Medicine of the University of Brescia, Italy. She is doing a PhD in Technology for Health at the department of Information Engineering at the University of Brescia, Italy. She spent several months at the Department of Biomedical Engineering at the University of Glasgow, Scotland during her PhD. Her main research interest is focused on the study of the human Mesenchymal Stem Cells applicationsin combination with biocompatible and bioresorbable scaffolds for a cost-effective regenerative therapy translatable into the clinical practice.