As it has been previously demonstrated that co-electroporation of Xenopus laevis frog oocytes with normal cells and cancerous cell lines induces the expression of pluripotency markers, and in experimental murine model studies that mRNA extract (Bioquantine purified from in-tra- and extra-oocyte liquid phases of electroporated oocytes) showed potential as a treatment for a wide range of conditions as Squint, Spinal Cord Injury (SCI) and Cerebral Palsy among others. The current study observed beneficial changes with Bioquantine administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative potential in clinical situations CNS disorders even cancer.

One method of reprogramming somatic cells into pluripotent stem cells is to expose them to extracts prepared from Xenopus laevis oocytes. We showed previously that coelectroporation of Xenopus laevis frog oocytes; with normal cells and cancerous cells lines, induces expression of markers of pluripotency. We also observed therapeutic effects of treatment with a purified ex-tract (Bioquantine) of intra- and extra-oocyte liquid phases derived from electroporated X. laevis oocytes, on experimentally induced pathologies including murine models of melanoma, traumatic brain injury, and experimental skin wrinkling induced by squalene-monohydroperoxide (Paylian et al, 2016). The positive human findings for spinal Cord Injury, and Cerebral Palsy with the results from previous animal studies with experimental models of traumatic brain injury, respectively (Paylian et al, 2016). Because of ethical reasons, legal restrictions, and a limited numbers of patients, we were able to treat only a very small number of patients. These results indicate that Bioquan-tine may be safe and well tolerated for use in humans, and deserves further study in a range of degenerative disorders. We propose that the mechanism of action of Bioquantine in these various diseases derives from its unique pharmacology and combinatorial reprogramming properties.

In conclusion, these preliminary findings suggest that Bioquantine is safe and well tolerated on patients with Cerebral Palsy and Spinal Cord Injury, among others. In addition to the regenerative therapy and due to the patient condition, we decided to include the Restore-Sensor Sure Scan. Based on the electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan, we designed an improved delivery method for the in situ application of MSCs and Bioquantine in combination with the Restore Sensor Sure Scan.

Conclusions: To the present day the patient who suffered a total section of spinal cord at T12-L1 shows an improvement in sensitivity, strength in striated muscle and smooth muscle connection, 11 months after the first therapy of cell regeneration and 3 month after the placement of Restore Sensor at the level of the lesion, the patient with a complete medullary section shows an evident improvement on his therapy of physical rehabilitation on crawling from front to back by himself and standing on his feet for the first time and showing a progressively important functionality on the gluteal and legs sensitivity1.

Biography:
Dr. Joel I Osorio is the CEO and Founder - Biotechnology and Regenerative Medicine at RegenerAge International, Vice President of International Clinical Development for Bioquark, Inc., and Chief Clinical Officer at ReAnima^™ Advanced Biosciences. Westhill University School of Medicine, Mexico. Joel I Osorio is an Advance Fellow by the American Board of Anti Aging and Regenerative Medicine (A4M). Dr. Joel was a Visiting scholar at University of North Carolina at Chapel Hill (Dermatology) He was a Fellow in Stem Cell Medicine by the American Academy of Anti-Aging Medicine and University of South Florida.