International Conference on Immunology and Immunotechnology
November 1-3, 2017 Barcelona, Spain
Genome Wide Transcriptome Analysis of IL-17 and STAT3 Loop Activation Psoriasiform Murine Model
1Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, China
2Beijing University of Chinese Medicine, China
Psoriasis is an autoimmune skin disease characterized by T lymphocytes activation and keratinocytes excessive proliferation and abnormal differentiation. Recently, T-helper 17 (Th17) cells and its related cytokines have been important influence factors in the pathology of psoriasis. Signal transducers and activators of transcription 3 (STAT3) over expression mice representing for keatinocytes proliferation. We tried to apply IL-17 (2µg / mouse) intradermal injection on STAT3 mice for 24 hours forming psoriasis-like lesions. The experiment was divided into three groups: wild-type (C57BL/6) mice, STAT3 mice and IL-17-treated STAT3. Illumumia sequence technique was applied to the dorsal skin of three groups (n=3). The results showed that 203 up regulated and 75 down regulated mRNAs were identified in IL-17A treated STAT3 mice compared to WT mice. The up-regulated included the members of S100A protein family such as S100A8 and S100A9, small proline-rich protein 2 (Sprr2) such as Sprr2e, Sprr2g and Sprr2d, late cornified envelope-3 (LCE) genes such as LCE3d, LCE3e and LCE3f. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis dispicted the De-genes annotated pathway including innate immune response, arachidonic acid metabolism, cytokine-cytokine receptor interaction, et al. Those were in accordance with the pathological characteristics of psoriatic patients. In conclusion, our study verified that IL-17 and STAT3 loop activation play a key role in the pathogenesis of psoriasis, and IL-17-treated STAT3 mice model could be used as an animal model for the study of psoriasis.
Xinran Xie is on-job doctorate, assistant researcher. The main research direction is the study of pathogenesis in psoriasis.