Madridge Journal of Cancer Study & Research

ISSN: 2640-5180

International Conference on Stem Cells and Cell Biology
December 6-7, 2018 Valencia, Spain

Comparative Efficacy of Sebastiania hispida and Byrsonima Crassifolia in the Neutralization of Muscle Damage Caused by Bothrops Moojeni

Doroty Mesquita Dourado1,2*, Rosemary Matias1, Jessica Muller2, Fiorela Farias Milanesi2, Bruna Zancanelli Fetter2, Daniele Vitorelli2 and Tatiane dos Santos Fernandes2

1Anhanguera University – UNIDERP, Brazil
2Federal University of Mato Grosso do Sul, Brazil

DOI: 10.18689/2640-5180.a3.006

Download PDF

Bothrops moojeni snake bite causes hemorrhage and myonecrosis, however the standard anti-venom therapy generally is ineffective in neutralizing the local effects. In this way, it is important to identify complementary therapies to the use of antivenom, such as medicinal plants. The objective was to compare the effectiveness of aerial parts of Sebastiania hispida and Byrsonima crassifolia to neutralize the muscle damage caused by B. moojeni. Wistar rats were used and was separated into 4 groups (n=4): Control (saline); Venom (Vbm); Venom and hydro-methanolic extract of S. hispida (VSh); Venom and water extract of B. crassifólia (VBc). The venom (40 μg/mL) or saline was injected in the gastrocnemius muscle of the right paw. The extracts were administered orally. After the periods of 24 hours, 3, 7 and 14 days, blood was collected for evaluation of creatine kinase (CK) and the gastrocnemius muscle was used for histological analysis. Vbm caused an increase in CK levels, indicating that there was muscle damage and only the VSh extract reduced CK levels. Muscle damage was confirmed by histological analysis, being observed necrosis, hemorrhage and edema in Vbm group. After 14 days the Vsh extract reduced hemorrhage, edema and necrosis, while Vcb only reduced the hemorrhage. Thus, medicinal plant extracts are great alternatives to counteract the local damage caused by B. moojeni and, among the two species analyzed, the extract of S. hispida was the most effective in neutralizing these effects.

Doroty Mesquita Dourado, PhD and postdoctoral studies from UNICAMP/SP. She works as a professor and researcher at University Anhanguera Uniderp in Campo Grande, MS. Has published more than 20 papers in reputed journals and has been serving as an editorial board member of repute. Their researches are carried out with medicinal plans of the Pantanal and Cerrado in the Laboratory of Toxinology and Medicinal Plants of the University Anhanguera Uniderp in Campo Grande, MS.