Madridge Journal of Cancer Study & Research

ISSN: 2640-5180

5th International Conference on Oncology & Virology
July 25-26, 2019 | Holiday Inn Rome Aurelia, Rome, Italy

Structure and Oligomerization State of the C-Terminal Region of the Middle East Respiratory Syndrome Coronavirus Nucleoprotein

Thi Hong Van Nguyen1,2*, Julie Lichière1,2, Bruno Canard1,2, Nicolas Papageorgiou1,2, Sarah Attoumani1,2, François Ferron1,2 and Bruno Coutard1,2

1Aix-Marseille Université, France
2CNRS, France

DOI: 10.18689/2640-5180.a4.008

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Middle East respiratory syndrome (MERS) is a positive single-stranded RNA virus belonging to lineage C of the genus Beta-Coronavirus within the Corona viridae family and the order Nidovirales (Chan et al., 2015). In 2015-2016, there was a largest outbreak seen in Middle East, and the Republic of Korea with the mortality rate up to 35%. MERS-CoV is a very dangerous respiratory disease, but currently, there is no vaccine or specific treatment to be available. Treatment is supportive and based on the patientʼs clinical condition (WHO, Jan 2018). Therefore, it is necessary to have more researches on it to find an antiviral drug for treatment. The nucleoprotein (N) structure of virus in the genus alpha-CoV, gamma-CoV and beta-CoV lineage B have identified, but the N structure of MERS-CoV has not identified yet. The Coronavirus nucleoprotein (CoV N) includes two folded domains called N-terminal domain (NTD) and C-terminal domain (CTD), separated by a linker region. CoV N has many functions, such as interacting with structural proteins; packaging the viral genome with structural proteins to form ribonucleo protein complexes; stopping host translation and making immune system interference. The main function of CTD nucleoprotein (N CTD) is a strong candidate for design of diagnostic tools (Ruth McBride et al., 2014; Chan et al., 2015).The structure of NTD was identified, but there is no structure for CTD and full length N MERS-CoV in order to support this functional hypothesis. Therefore, the aim of project is to characterize the N CTD structure of MERS-CoV using X-ray crystallography.

Biography:
Nguyen Thi Hong Van is a Vietnamese student. In 2014, she performed the 1st Master Internship in Hanoi University of Pharmacy, Vietnam. In 2015, she carried out the 2nd Master Thesis in Institute of Sophia Agrobiotech, Nice Sophia-Antipolis University, France. Then, she completed her Masters at the University of Sciences and Technologies of Hanoi, Vietnam and at the Co-University of Montpellier II, France. From 2016 until now, she has been a Doctoral student who has worked in Architecture et Fonction des Macromolécules Biologiques (AFMB), Marseille, France.

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