,,,,,,, Madridge Publishers | Journal of Biotechnology and Recent Advances

International Journal of Biotechnology and Recent Advances

ISSN: 2639-4529

European Microbiology Research Conference
December 3-4, 2018 Valencia, Spain

Analysis of Seven Putative Na+/H+ Antiporters of Arthrospira platensis Nies-39 using Transcription Profiling and in Silico Conserved Domain Studies: An Indication towards Alkaline pH Acclimation

Bhagavatula Vani*, Monika M. Jangir and Shibasish Chowdhury

Birla Institute of Technology and Science, India

DOI: 10.18689/2639-4529.a1.009

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Seven putative Na+/H+ antiporters have been reported to be present in A. platensis NIES-39 which thrives well even at pH 11. This raises a question whether all seven antiporters are equally involved in alkaline pH homeostasis in this cyanobacterial strain. To this end, we studied the transcriptional profile of these seven putative Na+/H+ antiporters. We found a significant difference in the m-RNA levels of the seven antiporters at pH 7, 9 and at 11. Also, a temporal pattern of the expression profile was observed. In silico conserved domain analysis of these seven putative antiporters indicated the presence of nine different kinds of domains in all, out of these nine domains, six domains function as monovalent cation-proton antiporters and two of the domains function as the universal stress protein (Usp) category which are UspA and Usp Like. The protein sequence of these putative antiporters have been compared and correlated with the solved crystal structure available from the CPA Super family (CPA1 & CPA2). The In-silico analysis and the real time PCR analysis, put together, suggest an active participation of these seven putative Na+/H+ antiporters in alkaline pH homeostasis of this cyanobacterial strain.

Bhagavatula Vani is presently working as Assistant Professor in Department of Biological sciences, BITS, Pilani, India. She has carried out her Doctoral in the field of abiotic stress and photosynthesis in Rice plants under the supervision of Late. Prof. Prasanna Mohanty, JNU, India who is an exponent of photosynthesis from India. She has published some of her works in journals like JPP, Photosynthetica, Plant and Soil and BiologiaPlantarum. She has also worked on nitrogen metabolism and abiotic stress in the wonder microbe, Arthrospira platensis (Spirulina platensis) and published some of the results.