International Journal of Biotechnology and Recent Advances

ISSN: 2639-4529

3rd International Biotechnology and Research Conference

October 23, 2020, Webinar
Scientific Session Abstracts
DOI: 10.18689/2639-4529.a3.002

Antimicrobial and Cytotoxic Effect of Nanostructured Lipid Carriers for Dermal Applications

Dragana P C de Barros1*, Patricia Reed1, Marta Alves1, Mariana G Pinho1 and Abel Oliva1,2

1Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Portugal
2Institute of Experimental Biology and Technology, Portugal

Nanostructured lipid carriers (NLC) as 2nd generation of lipid nanoparticles are promising systems to provide efficient skin delivery of therapeutic bioactive compounds. The use of vegetable oils in NLC formulations have numerous benefits for the skin due to their antioxidant, anti-inflammatory and antimicrobial activity. Many components naturally present in vegetable oils have been shown to have beneficial properties This work aimed to assess the influence of the structure of nanostructured lipid carriers (NLCs) containing vegetable (sunflower, olive, corn, peanut, coconut, castor and sweet almond) and essential (eucalyptus) oils on epidermal cells bioavailability and its antimicrobial activity against four Staphylococcus aureus strains. Different solid lipid (SL): liquid lipid (LL) ratios were used to study the structural properties and bioavailability of the particles. The choice of vegetable oil influenced physicochemical stability by changing the diameter of NLC formulations (between 160nm and 185nm) and z-potential (between -46mV and -61mV). Crystallinity of the lipid matrix was also influenced by chemical composition of different oils. All the systems (independent of the type of oil) were characterized by concentration-dependent cytocompatibility toward human epidermal keratinocyte (HaCaT) and human dermal fibroblasts (HDFn). The SL:LL ratio in NLC formulations showed higher impact on human dermal fibroblasts then on human epidermal keratinocyte, with no toxic effect on both cell lines. Moreover, antimicrobial properties were found in all 16 systems with the different grade of efficiency. These results pose a strong argument that the using natural oils in nanocarriers formulation can be explored as a promising carrier for skin treatment.

Dragana P C de Barros is senior research scientist, member of Biomolecular Diagnostic Laboratory at the Institute of Chemical and Biological Technology António Xavier, Portugal, with the scientific interest including the crossover between experimental and modelling studies in the area of bioprocess and cutaneous drug delivery systems development. She explores the potential of biodegradable lipid carriers based on natural compounds to capture polyphenolic compounds and the mechanism of transdermal delivery. DPC de Barros published 20 peer-reviewed scientific papers. She also has various communications published in the context of academic conferences, oral and poster communications in international and national conferences.

Differentiation between Three Indigenous Goat Types using Multivariate Analysis of Morphometric Traits in the Arganeraie of Agadir Region from Morocco

Houda El Kheyyat and Said El Madidi

Biometrics and Bio Resources, Laboratory BVRN, Faculty of Sciences, University Ibn Zohr, Morocco

The present study was carried out in the Arganeraie of Agadir in Morocco and consists of evaluating the differentiation between three goat type using multivariate analysis of morpho-biometric traits. The study involved 595 adult animals (539 females and 56 males) from 3 local goat breeds: Atlas, Barcha and Ghazalia. 14 Morphometric traits were used (9 quantitative variables and 6 qualitative variables). The results obtained showed the existence of a differentiation between the 3 local goat breeds with highly significant differences for CD,PW, LWandCR. The coefficient of variation of all characters in all 3 breeds ranged from 7% to 42% (CV between 6.39% for height at withers and 28.75% for length of horns). Principal component analysis (PCA) and discriminant factor analysis (DFA) shows a very significant difference between the three goat breeds and a high degree of intra-breed heterogeneity. The greatest Mahalanobis distance (17.21) was observed between the Atlas and Ghazalia, while the smallest distance (2.63) was recorded for the Atlas and Barcha. The cross-validation procedure assigned 67.1% of the Ghazalia animals to their genetic group, while the percentages of animals well classified in their original group for Atlas and Barchawere 100%.

Keywords: Arganeraie, local goat breeds, morpho-biometric traits, multivariate analysis, Morocco

Houda El Kheyyat, is pursuing her higher studies at the Faculty of Sciences, Ibn Zohr Agadir.

A Massive Gene Duplication of Hepcidin Family in a Mediterranean Bony Fish (Sparus aurata)

Jhon Alberto Serna Duque*, Alberto Cuesta and Maria Angeles Esteban

University of Murcia, Spain

At the present, infectious diseases limit the raise of European aquaculture and antibiotics are not allowed due to the many environmental and antimicrobial resistance problems arose due to their uncontrolled use in the past. To face with this challenge, antimicrobial peptides (AMPs) are postulated as a sustainable and safe therapeutic alternative to antibiotics for farmed fish. Hepcidins are a very widespread family of AMPs among vertebrates and very diverse in teleosts. It has delved into the identification of new hepcidins in gilthead seabream (Sparus aurata), a Mediterranean farmed fish. Gene sequence identification strategies, which are based on hidden Markov models (HMM) and ab initio gene predictions supported with ESTs, have been used to find new hamp genes in gilthead seabream genome. Results reveal a massively hamp duplicated genes. Fourteen hamp2-like gene copies and single hamp1 gene are located on chromosome 17 and they are supported with number copies quantification using qPCR in gDNA. All these new gilthead seabream hepcidins may have several biotechnological applications. Besides, we could speak now about a new world of hepcidins in perciform fish.

Tlf. 654282759

Acknowledgments: This work was supported by the MINECO co-funded by the European Regional Development Funds (ERDF/FEDER) (grant no. AGL2017-88370-C3-1-R) and by the Fundación Séneca de la Región de Murcia, Grupo de Excelencia, grant number 19883/GERM/15.

Jhon Alberto Serna Duque graduate in Biotechnology degree and in Master of Molecular Biology and Biotechnology of University in Murcia. He is the first year of PhD after getting a National grant (FPU) for four years of doctorate and received an award for the Best Innovative Project on the field of disruptive technology.

Modern Day Tools to Combat Modern World Challenges: Next Generation Sequencing FindingSolutions to COVID-19 Pandemic

Divya Vashisht

National Genomics Core-CDFD, India

Next generation sequencing (NGS) has been revolutionizing the modern day biology. In particular, during the unprecedented global scenario, NGS has quickly provided insight in identifying the solutions to combat ongoing pandemic. Using different NGS technologies, researchers have been able to understand the epidemiology and genomics of novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) causing COVID-19. In this direction, we have used two different sequencing technologies namely Sequencing-by-synthesis (SBS) by Illumina and Oxford Nanopore to sequence SARS-CoV-2 from samples collected from state of Telanagana, India. In our study, we have identified the dominant lineages present in the samples collected in state of Telangana, India, among all identified lineages of SARS-CoV-2. Interestingly, our results show that symptomatic cases of COVID-19 have lower viral load in comparison to asymptomatic cases. Using NGS analysis, further viral genomic variants have been identified in collected samples.

Divya Vashisht joined Pondicherry Central University for Masters in Biotechnology. For her doctorate research, she joined Utrecht University, The Netherlands and studied the genomic response of Arabidopsis to multiple stress conditions. She undertook bioinformatics training for the analysis of profiled transcriptome at University of California, Riverside. Subsequently, she joined Gregor Mendel Institute of Plant Molecular Biology, Vienna, Austria for post-doctoral research. In my research she elucidated the role of microRNA in embryo development. Having interest in genomics and ambition to advance scientific understanding with the use of NGS tools, she joined Genewiz, USA as Next Generation Sequencing marketing manager. Currently, she is working as CEO at National Genomics Core, CDFD, Hyderabad, India. NGC is the establishment of Department of Biotechnology, India to provide genomics solutions to all academic and industry users.

Ouabain Synergizes the Antimicrobial Activity of Aminoglycosides against Staphylococcus aureus

Antresh Kumar

Central University of Haryana, India

S taphylococcus aureus is opportunistic pathogen which often causes nosocomial and community related infections with rapid emerging resistance due to unseemly use of antibiotics and now become a serious health issue. Ouabain (OBN) is a Na+/K+-ATPase inhibitor that leads to increase the heart contraction in patients with congestive heart failure. In the present work, we determined antimicrobial effect of OBN together with aminoglycosides S. aureus strains. As compare to individual dose, OBN synergizes the staphylocidal potency by 16 (0.25μg/mL), 8 folds (0.5μg/mL) and 16 folds (1.0μg/mL) of Gentamycin (GEN), amikacin (AMK); kanamycin (KAN), respectively. The cell viability was completely lost within 60 min with GEN (1μg/mL), KAN (2μg/mL) and 90 min with AMK (1μg/mL). This bactericidal effect was enhanced due to GEN uptake potentiated by 66% which led to increase the cell permeability. The biofilm adherence disrupted by 80 and 50% at 5 mg/mL and 1.5 mg/mL OBN and 50 and 90% biofilm formation was inhibited at 5 mg/mL (MBIC50) and 10mg/mL (MBIC90), respectively. Moreover, OBN with GEN further induced biofilm inhibition by 67 ± 5% at pH 7.0. Taken together, we established that OBN synergizes the antimicrobial activity of aminoglycosides.

Antresh Kumar is Associate Professor, Department of Biochemistry, Central University of Haryana, Mahendergarh, India. His research is focused on to understand the pathogenic mechanism of Multidrug Drug Resistant (MDR) Staphylococcal and Candida related infections by employing Biochemical and transcriptional approaches. His research emphasized to find out a sustainable treatment either by using existing Therapeutics Molecule or by identifying new Antimicrobial Agents against S. aureus. Dr. Kumar has presented his work on different National and International platforms and also published his research in the peer-reviewed International Journals.