International Journal of Biotechnology and Recent Advances

ISSN: 2639-4529

International Biotechnology and Research Conference
April 25-27, 2018 Rome, Italy

Definition of Optimal In Vitro Conditions for Different Maturity Potato Cultivars

Maia Kukhaleishvili*, Ekaterine Bulauri, Tamar Shamatava, Tamar Chipashvili and Iveta Megrelishvili

Georgian Technical University, Biotechnology Center, Georgia

DOI: 10.18689/2639-4529.a1.003

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The research aim was to determine optimal in vitro condition for propagation different maturity potato cultivars. Potato varieties were collected from in vitro potato collection of Georgian Technical University, Biotechnology Center according to their maturity: Early cultivars: Viviana, Red Sonia, Bellarosa, Vineta, Anushka; Medium early: Donata, Bernina, Madeira, Sante, Laura. Medium late: Brodie, Shepody, Jelly, Carola, Desiree.

The influence of three types of combinations with temperature, humidity, light and photoperiod was studied on all three maturity potato cultivarʼs in vitro development:I. T-23-25° C, H-80%, Lux-5-5500, 16h; II. T-25-27° C, H-75%, Lux-5-5500,16h: III. T-27-29° C, H-70%, Lux-5-5500, 16h;The results were evaluated after 17 days of reproduction. Best in vitro condition for all researched potato varieties was selected for their leave colors, rooting, and shoot formation. All potato cultivars morphological characterization was variable depending on the type of in vitro condition. It was revealed early maturity cultivars had maximum potential for in vitro propagation (5-6 nodes, average rooting 92% and shoot formation 94%) on combination: T-23-25°C, H-80%, Lux-5-5500, 16h. Combination: T-25-27°C, H-75%, Lux-5-5500,16h was best for medium early cultivars (5-6 nods, rooting 89% and shoot formation 90%). The perfect in vitro reproduction of medium late varieties (5-6 nodes, average rooting 87% and shoot formation 82%) was observed on the combination: T-27-29°C, H-70%, Lux-5-5500, 16h.

As it is known, for in vitro developments of early medium and late medium potato cultivars are necessary 22-26 days, but our results were obtained in 17 days. Finally, optimal in vitro condition for propagation different maturity potato cultivars was defined.

Biography:
Maia Kukhaleishvili has completed his PhD at the age of 58 years from ST. Andrew the First Called Georgian University of the Patriarchate of Georgia. She is the director of Georgian Technical University, Biotechnology Center- Scientific-Research Center. She has published more than 10 papers in reputed journals and has a great experience in the field of Agriculture and Biotechnology.

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